USPTO Patents Application 10687850

PCX

s ^ ^ ^

(51) mm^ff^ms

COIN 27/327

Al

(11) BBS^PSSf- W099/51974

(43) mm^m 0 1999^10^ 14 0 (14.10.99)

(21) BKWjgS-g- PCT/JP99/01706

(22) SKWS B 1999^3^ 31 0 (31.03.99)

(30) s^mf-^

4#ffi¥lO/89740 1998^4^ 2 0(02.04.98) JP
«FH5pio/i72766 1998¥6^ 190 (19.06.98) JP

(71) WKA (*BS:^<•r^T^o^tSB^^:ov^T)

(MATSUSHITA ELECrrWC INDUSTWAL CO., LTD.)tJP/JPl
=f 571-8501 :^C^R*rtT:*:^^n^fl006»« Osaka, (JP)

(72) ; ^3j;t5

(75) mm^/mmx (*a^J:ov^•co*)

{t(IKEDA.Shm)[JP/JPJ • -
T576-0022 :^cBBfif2eif rfi»:ft^^T B 5-16-205 Osaka. (JP) , .

Jf(YOSHIOKA, Toshihiko)[JP/IP]
=f 573-0035 :;^fiKiff«::frmii5i^»ri5.15-307 Osaka, (JP)
*?S5t^(NANKAI. Shiro)[JP/JP]
T573-0071 :^cBUSft:;^mjS^<^TB 50-12 Osaka, (JP)

(74) i^mx

#S± ^^ft^UaSHn.Kazuo)

=f 541-0041 :*:BBff*IRm**K*lfe2TB3#6-§-

*SUJ*l:f>'l' :&#1#fF»«9f Osaka, (JP)

(81) mi^m CN, US, 0cW4*fF (AT. BE. CH, CY. DE. DK,
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(54)Title: SUBSTRATE DETERMININQ METHOD

A method fcfr determining the concentration of a
substrate in a san^le solution by using an electrode
system including a working electrode and a counter
electrode both (wovided on an electrically insulating base
and a reaction layer containing at least an oxidoreductase
and an electron carrier aiid provided on the electrode
system and by electrochemically measuring the reduced
amount of electron carrier produced by an enagrme
reacticm in tiie reaction i^ieiein a tfiird electrode
fat detecting an interference substance is provided
sepaately fiom tibe reaction layer, Ihe sapply of a sample
«>lutlQn is detected by detecting an electrical change
between the counter electrode and the third electrode, the
current flowing betweoi the counter electrode and fbc
third electrode is measured, the value of the current is
made a positive oror, thoeafter the application of
voltage between the counter electrode and the third
electrode is canceled, and a voltage for oxidizing the
reductant of the electron carrier is applied between the
working electrode and the counter electrode to measure
the current generated between bo&i electrodes. This
method is hardly influenced by mterfering substances
such as easily oxidized substances and enables highly
reliable substrate determination.

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10

INTERNATIONAL SEARCH REPORT

International application No,
PCT/JP99/01706

A. CMSSmCABON OF SUBJECT MATreR
Int. CI* G01N27/327

According to Internationa] Patent Qassification (IPQ or to both nationai dassification and IPC

B« FIELDS SEARCHED

Minimum documentation searched (dassification system followed by classification symbols)
Int.Cl* G01N27/327

Documentation searched other than minimum documentation to the extent that such documents are included in the fields searched

Jitsuyo Shinan Koho 1922-1996 Toroku Jltsxsyo Shinan Koho 1994-1999

Kbkai Jitsuyo Shinan Koho 1971-1999 Jitsi^ Shinan Toroku Koho 1996-1999

Electronic data base consulted during the international search (name of data base and» where practicable, search terms used)

C. DOCUMENTS CONSIDERED TO BE RELEVANT

Category*

Citation of document, with indication, where appropriate, of the relevant passages

Relevant to claim No.

X

JP, 8-320304, A (Matsushita Electric Industrial

1-7

Co., Ltd.)r

3 December, 1996 (03. 12. 96),

Full text ; Figs. 1 to 8

£ EP, 732406, A & OA, 2153350, A

& US, 5582697, A & US, 5650062, A

X

jp, 5-340915, A (Matsushita Electric Industrial

1-7

Co., Ltd.),

24 December, 1993 (24. 12. 93),

Full text ; Figs. 1 to 4

& EP, 537761, A & US, 5264103, A

& DE, 69221808, E

A

JP, 2-310457, A (Matsushita Electric Industrial

1-7

Co. , Ltd. ) ,

26 December, 1990 (26. 12. 90),

Full text ? Figs. 1 to. 5 (Family: none)

[x] Further documents are listed in the oonttnuatloo of BgkC See patent family annex.

•A'

•r

'V

'or

special categories of dted docomeats:

doGomem ddtfung the geoefai slate of the art wtiGb isnol

oonsidacd to be of paiticubr rdevanoe

earlier docwneat liui published on or after the intematicaal fifing dale
docomcm which may throw doobls on priority dainCs) of which is
died to esiabDsh the publicatio& date of another dlafioo or odier
special reason (as spedlied)

docitmem idoring ID aa oral (Ssciosore^ Qserexhibition or other

docnmeat poUided pnOT to the intcnafional fifing date but later than
the priori^ date cfaimed

"T" later docotncnt pabUshed after the intcniatioDa] Cling dale or pxkmty

date and oot In oonffict with the applicatioa but dted to mrfrfflanit

the priod|de or theory ooderiying &e invea^oa
Y docoiDemolpaiticQhu'relevantt;ihed»niedliiveati^

ooaskteied oovd or cannot be oolisideied to involve aa invealive step

vbeo the docnmeiit is nlcea ahme
*Y* docoiDemofparticohrrdeinmoe;lfaedaimedt]iveatf(»icaniiolbe

considered to involve aa inventive step when the doc ttflM Mt is

conMned with one or more other sDcli docomeais; socb oonibiiBtioB

being dbvioas to a posoa skiOcd in the ait
"A^ docnment member of the same patent family

Date of the actoai completion of the international seardi
23 June, 1999 (23. 06. 99)

Date of mailing of the international search report
13 July, 1999 (13. 07. 99)

Name and mailing address of the ISA/
Japanese Patent Office

Facsimile Na

Amborized officer
TdephooeNa

Fonn PCT/ISA/210 (second sheet) (JtUy 1992)

INTERNATIONAL SEARCH REPORT

International application No.
PCT/JP99/01706

C (Cbntinuation). DOCUMENTS CONSIDERED TO BE RELEVANT

Category*

Citation of document, with indication, where appropriate, of the relevant passages

JP, 9-101280, A (Casio Computer Co., Ltd.)/

15 April, 1997 (15. 04. 97),

Full text ; Figs. 1 to 5 (Family: none)

JP, 3-54447, A (Matsushita Electric Industrial Co.,
Ltd.),

8 March, 1991 (08. 03. 91),

Full text ; Figs. 1 to 3 (Family: none)

JP, 6-109693, A (Matsushita Electric Industrial
Co. , Ltd. ) ,

22 April, 1994 (22. 04. 94),

Full text ; Figs. 1 to 3 (Family: none)

JP, 10-19832, A (NOK Corp.),

23 January, 1998 (23. 01. 98),
Full text ; Fig. 1 ( Family t none)

JP, 9-201337, A (Casio Computer Co., Ltd.),

5 August, 1997 (05. 08. 97),

Full text ; Figs. 1 to 6 (Family: none)

Relevant to daim Na

1-7

1-7

1-7

1-7

1-7

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